T4 DNA Polymerase from New England Biolabs

Product Specs
ItemT4 DNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0203S
Quantity150 units
Concentration3 U/μl
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is the amount of enzyme which incorporates 10 nmoles of dNTP into acid precipitable material in 30 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Rapid and Quantitative Functional Interrogation of Human Enhancer Variant Activity in Live MicebioRxivDecember 10, 2023Ethan W. Hollingsworth, Taryn A. Liu, Sandra H. Jacinto, Cindy X. Chen, Joshua A. Alcantara, Evgeny Z. Kvon(100 μM; Jena Bioscience, NU-831-BIOX) were added to the reaction with T4 DNA Polymerase (1 unit; NEB, M0203) and incubated at 12°C for 15 min. The reaction was stopped by addition of EDTA (100 mM) and heat inactivated

(2) Genome folding principles revealed in condensin-depleted mitotic chromosomesbioRxivNovember 17, 2023Han Zhao, Yinzhi Lin, En Lin, Fuhai Liu, Lirong Shu, Dannan Jing, Baiyue Wang, Manzhu Wang, Fengnian Shan, Lin Zhang, et al.re-suspended in 30μl of 1 × NEB Buffer2 (NEB Cat#7002S) containing 9U of T4 DNA polymerase (NEB, Cat#M0203S) and 40μM dATG/dGTP and incubated at 20°C for 4 hours to remove unligated fragments. Beads were then

(3) Synthetic chromosome fusion: Effects on mitotic and meiotic genome structure and functionCell GenomicsNovember 8, 2023Jingchuan Luo, Luis A. Vale-Silva, Adhithi R. Raghavan, Guillaume Mercy, Jonna Heldrich, Xiaoji Sun, Mingyu Kenneth Li, Weimin Zhang, Neta Agmon, Kun Yang, et al.,New England BioLabs,M0201S Klenow Fragment,New England BioLabs,M0210S T4 DNA Polymerase,New England BioLabs,M0203S dATP (100 mM),ThermoFisher Scientific,10216018 Klenow Fragment exo-,New England BioLabs,M0212S Agencourt

(4) Extrachromosomal Telomeres Derived from Excessive Strand DisplacementsbioRxivOctober 27, 2023Junyeop Lee, Jina Lee, Eric J. Sohn, Angelo Taglialatela, Roderick J. O’Sullivan, Alberto Ciccia, Jaewon MinNylon Membranes-positively charged, (Roche, 11417240001), exo‒ Klenow fragment (NEB, M0212), T4 DNA polymerase (NEB, M0203), Lambda exonuclease (NEB, M0262), DIG-dUTP (biorbyt, orb533128), NxGen phi29 (Biosearch Technologies,

(5) Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanomaiScienceOctober 20, 2023Eliot Y. Zhu, Jacob L. Schillo, Sarina D. Murray, Jesse D. Riordan, Adam J. Dupuy#: R3136S Enzyme: dsDNA Fragmentase,NEB,Catalog #: M0348 Enzyme: T4 DNA polymerase,NEB,Catalog #: M0203 Enzyme: T4 DNA ligase,NEB,Catalog #: M0202 ,, Deposited data,Deposited data,Deposited data ,, Raw

(6) Regulation of CTCF loop formation during pancreatic cell differentiationNature CommunicationsOctober 9, 2023Xiaowen Lyu, M. Jordan Rowley, Michael J. Kulik, Stephen Dalton, Victor G. Corcesresuspended in 1x NEBuffer 2.1 (NEB, #B7202S) and dephosphorylated by the addition of 5 μl rSAP (NEB, #M0203) at 37 °C for 45 min. 5′ overhangs were generated with the following pre-mix (50 mM NaCl, 10 mM Tris,

(7) Uncoupling the TFIIH Core and Kinase Modules Leads To Unregulated RNA Polymerase II CTD Serine 5 PhosphorylationbioRxivSeptember 16, 2023Gabriela Giordano, Robin Buratowski, Célia Jeronimo, Christian Poitras, François Robert, Stephen Buratowskiwere repaired by incubating in 70 μL of 1X NEBuffer 2 containing 0.6 units of T4 DNA polymerase (NEB, M0203S), 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for

(8) High-coverage genome of the Tyrolean Iceman reveals unusually high Anatolian farmer ancestryCell GenomicsSeptember 13, 2023Ke Wang, Kay Prüfer, Ben Krause-Kyora, Ainash Childebayeva, Verena J. Schuenemann, Valentina Coia, Frank Maixner, Albert Zink, Stephan Schiffels, Johannes Krause,Thermo Fisher Scientific,Cat# 4379874 dNTP Mix,Thermo Fisher Scientific,Cat# R1121 T4 DNA Polymerase,New England Biolabs,Cat# M0203 T4 Polynucleotide Kinase,New England Biolabs,Cat# M0201 Bst 2.0 DNA Polymerase,New England Biolabs,Cat#

(9) The extinct Sicilian wolf shows a complex history of isolation and admixture with ancient dogsiScienceAugust 18, 2023Marta Maria Ciucani, Jazmín Ramos-Madrigal, Germán Hernández-Alonso, Alberto Carmagnini, Sabhrina Gita Aninta, Xin Sun, Camilla Hjorth Scharff-Olsen, Liam Thomas Lanigan, Ilaria Fracasso, Cecilie G. Clausen, et al.T4 Polynucleotide Kinase,New England Biolabs Inc.,Cat#M0201L T4 DNA Polymerase,New England Biolabs Inc.,Cat#M0203S "BSt 2,0 warmstart polymerase",New England Biolabs Inc.,Cat#M0538S Phusion® High-Fidelity PCR Master Mix with HF buffer

(10) Aub, Vasa and Armi localization to phase separated nuage is dispensable for piRNA biogenesis and transposon silencing in DrosophilabioRxivJuly 29, 2023Samantha Ho, Nicholas P Rice, Tianxiong Yu, Zhiping Weng, William E Theurkauf,ThermoFisher Scientific,Cat# R0133 dNTP mix,NEB,Cat# N0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat# M0210S T4 PNK,NEB,Cat# M0201L Klenow 3ʹ to 5ʹ exo,NEB,Cat# M0212L UDG,NEB,Cat#

(11) An upgraded method of high-throughput chromosome conformation capture (Hi-C 3.0) in cotton (Gossypium spp.)Frontiers in Plant ScienceJuly 4, 2023Jin Han, Siyuan Wang, Hongyu Wu, Ting Zhao, Xueying Guan, Lei FangT4 DNA ligase (NEB, M0202S); proteinase K (NEB, P8107S); RNase A (Biosharp, BL543A); T4 DNA polymerase (NEB, M0203S); DpnII (NEB, R0543S); DdeI (NEB, R0175S); NEBuffer 3 (NEB, B7003S). 2.2.2 Kits NEBNext Ultra II DNA Library Prep

(12) Histone exchange sensors reveal variant specific dynamics in mouse embryonic stem cellsNature CommunicationsJune 26, 2023Marko Dunjić, Felix Jonas, Gilad Yaakov, Roye More, Yoav Mayshar, Yoach Rais, Ayelet-Hashahar Orenbuch, Saifeng Cheng, Naama Barkai, Yonatan StelzerdGTP, dTTP), 1.25 μl of T4 polynucleotide kinase (NEB cat#M0201) and 0.167 μl of T4 polymerase (NEB cat#M0203). The samples were incubated 22 min at 12 °C followed by 22 min at 25 °C. After incubation, the samples

(13) Affinity-enhanced RNA-binding domains as tools to understand RNA recognitionCell Reports MethodsJune 26, 2023Belén Chaves-Arquero, Katherine M. Collins, Giancarlo Abis, Geoff Kelly, Evangelos Christodoulou, Ian A. Taylor, Andres RamosInternational,84609.0500 Sac1,New England Biolabs,R3156 Kpn1,New England Biolabs,R3142 T4 DNA polymerase,New England Biolabs,M0203 DpnI,New England Biolabs,R0176 KOD polymerase hot start master mix,Merck,71842 Magnesium sulfate,Sigma-Aldrich,208094 "Sodium

(14) A multiple super-enhancer region establishes inter-TAD interactions and controls Hoxa function in cranial neural crestNature CommunicationsJune 5, 2023Sandra Kessler, Maryline Minoux, Onkar Joshi, Yousra Ben Zouari, Sebastien Ducret, Fiona Ross, Nathalie Vilain, Adwait Salvi, Joachim Wolff, Hubertus Kohler, et al.on ice with 50 µL 2.5 mM dNTP mix (12.5 μL of 10 mM of each dNTP), 18 μL of T4 DNA Polymerase (NEB M0203), 18 μL of T4 PNK (NEB M0201), 3.7 μL of DNA polymerase I large fragment (Klenow; NEB M0210), and 360.1

(15) A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryosSTAR ProtocolsJune 1, 2023Daniel Gimenez-Llorente, Marta Portela, Ana Losada, Miguel Manzanares, Maria Jose Andreupolynucleotides kinase,New England Biolabs,Cat# M0201L T4 DNA polymerase,New England Biolabs,Cat# M0203L Klenow Fragment 3'->5′ exo-,New England Biolabs,Cat# M0212S Quick Ligation Buffer 5x,New England Biolabs,Cat#

(16) Regulatory architecture of housekeeping genes is driven by promoter assembliesCell reportsMay 30, 2023Dejosez M, Dall'Agnese A, Ramamoorthy M, Platt J, Yin X, Hogan M, Brosh R, Weintraub AS, Hnisz D, Abraham BJ, et al.Preparation protocol (Illumina).22 ChIP DNA fragments were end-repaired using T4 DNA polymerase (NEB, M0203) and A-tailed with Klenow (NEB, M0212). A biotinylated bridge linker (F:/5Phos/CGC GAT ATC/iBiodT/TAT

(17) Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variantsCell ReportsMay 30, 2023Jianbo Wu, Zhenguo Chen, Yidan Gao, Zegen Wang, Jiarong Wang, Bing-Yu Chiang, Yunjiao Zhou, Yuru Han, Wuqiang Zhan, Minxiang Xie, et al.Phusion High-Fidelity DNA Polymerase,Thermo Scientific,Cat#M0530L T4 DNA polymerase,New England BioLabs,Cat#M0203L T4 DNA ligase,New England BioLabs,Cat#M0202M NucleoSpin Gel and PCR Clean-up,New England BioLabs,Cat#740690.250 Luciferase

(18) A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regionsNucleic Acids ResearchMay 22, 2023Chen Pan, Ran Li, Liyan Shui, Zhengyun Xiao, Yali Wang, Jing Zhu, Chao Wu, Long Zhang, Junling Jia, Min Zhengdigested with BbsI (NEB, R3539). The 5′ overhang after BbsI cleavage was filled by T4 DNA polymerase (NEB, M0203), and then A4 adapter was ligated to the repaired library. The final gRNA library was amplified and

(19) Single duplex DNA sequencing with CODEC detects mutations with high sensitivityNature GeneticsMay 1, 2023Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea, et al.presence of 0.05 µg µl−1 BSA at 37 °C for 30 min. In step 2, T4 PNK (NEB, M0201S), T4 DNA polymerase (NEB, M0203S), 0.8 mM ATP and 0.5 mM dNTP mix are added into the step 1 reaction mix and incubated at 37 °C for another

(20) Disruption of the topologically associated domain at Xp21.2 is related to 46, XY gonadal dysgenesisJournal of Medical GeneticsMay 1, 2023Jakob A Meinel, Verónica Yumiceba, Axel Künstner, Kristin Schultz, Nathalie Kruse, Frank J Kaiser, Paul-Martin Holterhus, Alexander Claviez, Olaf Hiort, Hauke Busch, et al.(Thermo Fisher Scientific, 65602). The DNA ends were repaired using 12 U of T4 DNA polymerase (NEB, M0203), 5U of Klenow fragment of DNA polymerase I (NEB, M0210) and 50 U of T4 Polynucleotide Kinase (NEB,

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